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hepatitis b surface antigen ab elisa kit  (Abnova)

 
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    Abnova hepatitis b surface antigen ab elisa kit
    Hepatitis B Surface Antigen Ab Elisa Kit, supplied by Abnova, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepatitis b surface antigen ab elisa kit/product/Abnova
    Average 90 stars, based on 1 article reviews
    hepatitis b surface antigen ab elisa kit - by Bioz Stars, 2026-02
    90/100 stars

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    (B and C) HepG2.2.15 cells and HepG2 cells at the same cellular concentration (1 × 10 6 cells) were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively. (E and F) After the gradient transfection of pBlue-HBV 1.3-fold plasmids into HepG2 cells, the cells were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively, with the mRNA expression level of GAPDH as a reference. (H and I) Huh7 cells were analyzed in the same way as HepG2 cells. (A, D, and G) ELISA results for <t>HBeAg</t> and HBsAg concentrations in cell supernatants demonstrated successful infection.
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    Average 90 stars, based on 1 article reviews
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    (B and C) HepG2.2.15 cells and HepG2 cells at the same cellular concentration (1 × 10 6 cells) were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively. (E and F) After the gradient transfection of pBlue-HBV 1.3-fold plasmids into HepG2 cells, the cells were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively, with the mRNA expression level of GAPDH as a reference. (H and I) Huh7 cells were analyzed in the same way as HepG2 cells. (A, D, and G) ELISA results for HBeAg and HBsAg concentrations in cell supernatants demonstrated successful infection.

    Journal: PLOS ONE

    Article Title: Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

    doi: 10.1371/journal.pone.0312773

    Figure Lengend Snippet: (B and C) HepG2.2.15 cells and HepG2 cells at the same cellular concentration (1 × 10 6 cells) were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively. (E and F) After the gradient transfection of pBlue-HBV 1.3-fold plasmids into HepG2 cells, the cells were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively, with the mRNA expression level of GAPDH as a reference. (H and I) Huh7 cells were analyzed in the same way as HepG2 cells. (A, D, and G) ELISA results for HBeAg and HBsAg concentrations in cell supernatants demonstrated successful infection.

    Article Snippet: The ELISA kits for hepatitis B surface antigen (HBsAg) and e-antigen (HBeAg) detection were purchased from Shanghai Kehua Biological Engineering Co., Ltd., and the ELISA kit for QPCT detection was purchased from SAB (Signalway Antibody LLC).

    Techniques: Concentration Assay, Cell Culture, Expressing, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Infection

    (A and D) HBV 1.3-fold plasmids and various concentrations of PcDNA3.1–3*Flag-QPCT were cotransfected into Huh7 and HepG2 cells and cultured for 48 h, supernatants and cells were harvested. (A and D) The overexpression of Flag-QPCT in the transfected HepG2 and Huh7 cells was detected by Western blot. (B and E) The expression levels of HBeAg and HBsAg in the supernatants were detected by ELISA. (C and F) Total RNA was extracted from the cells, and the mRNA expression of HBV pgRNA in the cells was detected by RT‒PCR, with the mRNA expression level of GAPDH as a reference. (G and H) Total DNA was extracted from the cells, and the HBV-DNA copies in Huh7 and HepG2 cells were detected by RT‒PCR.

    Journal: PLOS ONE

    Article Title: Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

    doi: 10.1371/journal.pone.0312773

    Figure Lengend Snippet: (A and D) HBV 1.3-fold plasmids and various concentrations of PcDNA3.1–3*Flag-QPCT were cotransfected into Huh7 and HepG2 cells and cultured for 48 h, supernatants and cells were harvested. (A and D) The overexpression of Flag-QPCT in the transfected HepG2 and Huh7 cells was detected by Western blot. (B and E) The expression levels of HBeAg and HBsAg in the supernatants were detected by ELISA. (C and F) Total RNA was extracted from the cells, and the mRNA expression of HBV pgRNA in the cells was detected by RT‒PCR, with the mRNA expression level of GAPDH as a reference. (G and H) Total DNA was extracted from the cells, and the HBV-DNA copies in Huh7 and HepG2 cells were detected by RT‒PCR.

    Article Snippet: The ELISA kits for hepatitis B surface antigen (HBsAg) and e-antigen (HBeAg) detection were purchased from Shanghai Kehua Biological Engineering Co., Ltd., and the ELISA kit for QPCT detection was purchased from SAB (Signalway Antibody LLC).

    Techniques: Cell Culture, Over Expression, Transfection, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

    (B and C) HepG2.2.15 cells and HepG2 cells at the same cellular concentration (1 × 10 6 cells) were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively. (E and F) After the gradient transfection of pBlue-HBV 1.3-fold plasmids into HepG2 cells, the cells were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively, with the mRNA expression level of GAPDH as a reference. (H and I) Huh7 cells were analyzed in the same way as HepG2 cells. (A, D, and G) ELISA results for HBeAg and HBsAg concentrations in cell supernatants demonstrated successful infection.

    Journal: PLOS ONE

    Article Title: Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

    doi: 10.1371/journal.pone.0312773

    Figure Lengend Snippet: (B and C) HepG2.2.15 cells and HepG2 cells at the same cellular concentration (1 × 10 6 cells) were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively. (E and F) After the gradient transfection of pBlue-HBV 1.3-fold plasmids into HepG2 cells, the cells were cultured for 48 h. Supernatants and cells were harvested. The protein and mRNA expression levels of QPCT were detected by Western blot and RT‒PCR, respectively, with the mRNA expression level of GAPDH as a reference. (H and I) Huh7 cells were analyzed in the same way as HepG2 cells. (A, D, and G) ELISA results for HBeAg and HBsAg concentrations in cell supernatants demonstrated successful infection.

    Article Snippet: The ELISA kits for hepatitis B surface antigen (HBsAg) and e-antigen (HBeAg) detection were purchased from Shanghai Kehua Biological Engineering Co., Ltd., and the ELISA kit for QPCT detection was purchased from SAB (Signalway Antibody LLC).

    Techniques: Concentration Assay, Cell Culture, Expressing, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Infection

    (A and D) HBV 1.3-fold plasmids and various concentrations of PcDNA3.1–3*Flag-QPCT were cotransfected into Huh7 and HepG2 cells and cultured for 48 h, supernatants and cells were harvested. (A and D) The overexpression of Flag-QPCT in the transfected HepG2 and Huh7 cells was detected by Western blot. (B and E) The expression levels of HBeAg and HBsAg in the supernatants were detected by ELISA. (C and F) Total RNA was extracted from the cells, and the mRNA expression of HBV pgRNA in the cells was detected by RT‒PCR, with the mRNA expression level of GAPDH as a reference. (G and H) Total DNA was extracted from the cells, and the HBV-DNA copies in Huh7 and HepG2 cells were detected by RT‒PCR.

    Journal: PLOS ONE

    Article Title: Increased QPCT gene expression by the hepatitis B virus promotes HBV replication

    doi: 10.1371/journal.pone.0312773

    Figure Lengend Snippet: (A and D) HBV 1.3-fold plasmids and various concentrations of PcDNA3.1–3*Flag-QPCT were cotransfected into Huh7 and HepG2 cells and cultured for 48 h, supernatants and cells were harvested. (A and D) The overexpression of Flag-QPCT in the transfected HepG2 and Huh7 cells was detected by Western blot. (B and E) The expression levels of HBeAg and HBsAg in the supernatants were detected by ELISA. (C and F) Total RNA was extracted from the cells, and the mRNA expression of HBV pgRNA in the cells was detected by RT‒PCR, with the mRNA expression level of GAPDH as a reference. (G and H) Total DNA was extracted from the cells, and the HBV-DNA copies in Huh7 and HepG2 cells were detected by RT‒PCR.

    Article Snippet: The ELISA kits for hepatitis B surface antigen (HBsAg) and e-antigen (HBeAg) detection were purchased from Shanghai Kehua Biological Engineering Co., Ltd., and the ELISA kit for QPCT detection was purchased from SAB (Signalway Antibody LLC).

    Techniques: Cell Culture, Over Expression, Transfection, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay